Systematic review of external applications combined with three-step analgesic therapy in treating primary liver cancer pain / 中国中药杂志

To systemically evaluate the clinical efficacy and safety of traditional Chinese medicine( TCM) external application combined with three-step analgesic therapy in treating primary liver cancer pain. CNKI,Wanfang,CBM,VIP,Medline and Cochrane Library and manual retrieval were used to search for the clinical randomized controlled trials on TCM external applications combined with three-step analgesic therapy in treating primary liver cancer pain from database establishment to January,2018. The bias risk of RCTs was assessed by using the Cochrane system evaluator's Manual,and the extracted data were analyzed by using Review Manager 5. 3. Finally sixteen Chinese articles were enrolled,including one high quality article and 1 164 patients. Meta-analysis showed that TCM external applications combined with three-step analgesic therapy could alleviate the cancer pain( OR = 3. 44,95% CI[2. 49,4. 75],P <0. 000 01); prolong pain relief time( SMD = 3. 42,95%CI[1. 83,6. 40],Z = 3. 85,P = 0. 000 1); and improve the cartesian score of the patients( OR = 3. 42,95%CI[1. 83,6. 40],P = 0. 000 01). Descriptive analysis showed that the intervention may effectively shorten the onset time of pain relief,reduce VAS and NRS scores,reduce the dose of morphine,and reduce the number of bursts of pain. At present,the evidences have shown that the combination of TCM external applications combined with three-step analgesic therapy in treating primary liver cancer pain has superior clinical efficacy as compared with the three-step analgesic therapy alone. However,the clinical trials of existing small-sized randomized controlled trials have low quality of methodology and require a large sample of high quality clinical trials for further validation.

Investigation and evaluation of disease associated nutrition knowledge, diet attitude and practice in patients with liver failure / 肠外与肠内营养

Objective:Based on the evaluation of the disease associated nutrition knowledge,diet attitude and practice (KAP) in patients with liver failure this study aimed to identify factors that correlated with KAP level,and to provide evidence for the disease specific nutritional education programs.Methods:In this study,178 patients with liver failure were randomized selected from a tertiary hospital between October 2015 and February 2017.Liver failure disease associated nutrition knowledge,diet attitude and practice were assessed through questionnaire.Results:The average score of disease nutrition KAP items in patients with liver failure wes (1.56 ± 0.44) points,with the highest average score of diet attitude was (1.80 ± 0.50) and the lowest average score of nutrition knowledge was (1.39 ± 0.60) points.There was a significant negative correlation between the KAP scores and the PG-SGA classification (P ＜ 0.01).Education level,family income,disease status are risk factor of disease nutrition KAP in patients with liver failure.Conclusion:Patients with liver failure are characterized by limited nutritional knowledge,active diet attitude,and moderate diet behavior.The improvement of nutrition knowledge,dietary attitude and behavior level in patients with liver failure is important.Those with lower level of education,lower income and shorter duration of disease should be given more attention.

Effects of laparoscope assisted total gastrectomy and open gastrectomy combined with D2 dissection in the treatment of upper gastric cancer and the impact on stress response / 中国内镜杂志

Objective?To investigate the effects of laparoscope assisted total gastrectomy (LATG) and open total gastrectomy (OTG) combined with D2 dissection in the treatment of upper gastric cancer and the impact on stress response.?Methods?145 patients with upper gastric cancer who underwent total gastrectomy from June 2014 to June 2017 were selected as research subjects, and they were divided into study group (treated by LATG, n = 70) and control group (treated by OTG + D2 dissection, n = 75) according to different surgical methods. The general condition of surgery, stress indexes cortisol (Cor), C reactive protein (CRP), postoperative recovery and the incidence of complications were compared between the two groups.?Results?The surgical time of the study group was longer than that of the control group, while the length of incision, intraoperative blood loss and length of hospital stay were shorter/less than those of the control group (P < 0.05). There was no significant difference in the number of dissected lymph nodes between the two groups (P > 0.05). Serum Cor and CRP levels in the study group were lower than those the control group during surgery and at 3 d after surgery (P < 0.05). The initial exhaust time and recovery time of liquid diet in the study group was shorter than that in the control group (P < 0.05), but there was no significant difference between the two groups in the rate of second surgery and readmission rate within 30 days (P > 0.05). The incidence of complications in the study group at 6 months after surgery was lower than that in the control group (P < 0.05).?Conclusion?Compared with OTG and D2 dissection, LATG has advantages of less trauma, earlier exhaust and feeding and fewer stress reactions, with quick recovery and few complications.

Development and application of reference materials containing mixed degradation products of amoxicillin and ampicillin / 药学学报

Reference materials containing mixed degradation products of amoxicillin and ampicillin were developed after optimization of preparation processes. The target impurities were obtained by controlled stress testing, and each major component was identified with HPLC-MS and compared with single traceable reference standard each. The developed reference materials were applied to system suitability test for verifying HPLC system performed in accordance with set forth in China Pharmacopeia and identification of major impurities in samples based on retention and spectra information, which have advantages over the methods put forth in foreign pharmacopoeias. The development and application of the reference materials offer an effective way for rapid identification of impurities in chromatograms, and provide references for analyzing source of impurities and evaluation of drug quality.

Application of combined fluorescence in situ hybridization and karyotype analysis for the diagnosis of Robertsonian translocation type trisomy 21 / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To assess the value of fluorescence in situ hybridization (FISH) combined with chromosomal analysis for the detection of Robertsonian translocation type trisomy 21 in amniotic fluid cells.</p><p><b>METHODS</b>Amniotic fluid samples from pregnant women requesting prenatal diagnosis were cultivated. Metaphase cells were prepared for G-banding karyotype analysis. For the 5 Robertsonian translocation type trisomy 21, interphase nuclei from amniotic fluid and parental peripheral blood cells were prepared for FISH analysis.</p><p><b>RESULTS</b>In 2 cases, analysis of parental peripheral blood cells showed normal karyotypes. FISH analysis of amniotic fluid cells indicated that one sample had two copies of chromosome 21, which has a 46, XY, rob(21;21)(q10;q10) karyotype, whilst another had trisomy 21 by FISH, which has a 46, XY, rob(14;21)(q10;q10) karyotype. For the remaining three samples, analysis of parental peripheral blood cells indicated that their karyotypes were 45, XX, rob(14;21)(q10;q10), 45, XX, rob(15;21)(q10;q10) and 45, XX, rob(21;22)(q10;q10), whilst the karyotypes of amniotic fluid cells were 46, XX, rob(14;21)(q10;q10), 46, XY, rob(15;21)(q10;q10) and 46, XX, rob(21;22)(q10;q10), respectively.</p><p><b>CONCLUSION</b>Combined FISH and chromosomal analysis is an efficient method for detecting non-homologous Robertsonian translocation type trisomy 21. However, FISH has limited ability to detect homologous Robertsonian translocation type trisomy 21.</p>

Frozen embryo transfer combined with intrauterine administration of autologous peripheral blood mononuclear cells for repeated implantation failure: report of 3 cases / 南方医科大学学报

<p><b>OBJECTIVE</b>To explore the efficacy of frozen-thawed embryo transfer combined with intrauterine administration of autologous peripheral blood mononuclear cells (PBMCs) in the treatment of repeated implantation failure (RIF).</p><p><b>METHODS</b>PBMCs obtained from 3 patients with RIF on the day of follicle rupture (natural cycle) or when the endometrial thickness reached 8 mm (hormone replacement cycle) were cultured in the presence of HCG for 48 h. The cultured PBMCs, along with freshly isolated PBMCs, were administered into the uterine cavity of the patients. Vitrified cleavage-stage embryos or blastocysts transfer was performed on day 3 or 5, respectively.</p><p><b>RESULTS</b>Vitrified embryo or blastocyst transfer resulted in pregnancy and healthy live births in all the 3 patients.</p><p><b>CONCLUSION</b>Frozen-thawed embryo transfer combined with intrauterine administration of autologous PBMCs may be an effective and safe approach to the treatment of RIF and may improve the outcomes of assisted reproduction.</p>

In vitro fertilization versus intracytoplasmic sperm injection for primary and secondary infertility using sibling oocytes: clinical analysis of the outcomes / 南方医科大学学报

<p><b>OBJECTIVE</b>To evaluate the clinical outcomes of intracytoplasmic sperm injection (ICSI) and conventional in vitro fertilization (IVF) using sibling oocytes for treatment of primary and secondary infertility.</p><p><b>METHODS</b>A total of 149 cycles of IVF and ICSI were conducted between January, 2003 and December, 2008 in our center, including 98 cycles in patients with primary infertility and 51 in those with secondary infertility. According to the embryos derived from ICSI, IVF and their combination, the clinical pregnancy rate, delivery rate and birth defect of the 3 groups were analyzed.</p><p><b>RESULTS</b>The fertilization failure rate of IVF was significantly higher in primary infertility group than in secondary infertility group (10.2% vs 3.9%, P<0.05). No fertilization failure occurred in ICSI group. The fertilization rates and good quality embryo rates in ICSI group were significant higher than those in IVF group, and the abnormal fertilization rate was significantly lower in ICSI group (P<0.05). No significant difference were found in the implantation rates, clinical pregnancy rates, delivery rates or the rates of birth defects of the offsprings between IVF, ICSI and IVF+ICSI groups.</p><p><b>CONCLUSION</b>IVF combined with ICSI may result in increased fertilization rate and avoid total fertilization failure with favorable clinical outcomes in patients with long-term infertility, and ICSI may not increase the birth defects of the offspring in these patients.</p>

Theory of selectivity of RP-LC C18 column and its application / 药学学报

In recent years, more and more researchers focus on the selectivity of RP-HPLC columns. Among these columns, C18 column is the most commonly used the column. Yet even the same filler can not give us the same separation effect with the columns of different brands and different types, therefore, it is important to choose the suitable HPLC column for carrying out an experiment. After study in recent years, some column classification, selection methods (e.g.: column parameter method, F value method) have been achieved and contributed to the finding of suitable columns and reducing the blindness of column selection. This paper summarizes the advantages of these methods and their limitations in the application process, and gives some advice and expectations to pharmaceutical analysis works based on the works of other researchers.

Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry for proteomic analysis of seminal plasma in patients with delayed semen liquefaction / 南方医科大学学报

<p><b>OBJECTIVE</b>To identify the differentially expressed proteins in seminal plasma between subjects with normal fertility and those with delayed semen liquefaction by proteomic techniques.</p><p><b>METHODS</b>Semen samples of 6 patients with delayed semen liquefaction and 11 subjects with normal fertility (control group) were collected. Seminal plasma were separated and examined with surface-enhanced laser desorption/ionization time of flight mass spectrometry to compare the protein expressions between the two groups.</p><p><b>RESULTS</b>Compared with normal control group, 19 proteins were differentially expressed in the patients including 6 proteins with significant differences between the two groups, with m/z of 8696.621, 9770.076, 9512.309, 10202.64, 2941.903, and 9617.759, respectively (P<0.01).</p><p><b>CONCLUSION</b>Screening of differentially expressed proteins in seminal plasma may help understand the mechanism of delayed semen liquefaction for its potential clinical intervention.</p>

The biotransformation of kaempferitrin by human intestinal flora / 药学学报

<p><b>AIM</b>To study the biotransformation of kaempferitrin, a major chemical principle of the fruits of Siraitia grosvenori (Swingle) C. Jeffery, with human intestinal flora.</p><p><b>METHODS</b>The kaempferitrin was incubated with human intestinal flora. The biotransformation products were isolated and purified by chromatographic methods and the structures were determined by spectroscopic techniques.</p><p><b>RESULTS</b>Kaempferitrin was converted into kaempferol 3-O-alpha-L-rhamnoside (afzelin, I) , kaempferol 7-O-alpha-L-rhamnoside (II), kaempferol (III) and p-hydroxybenzoic acid (IV) by human intestinal flora. rhamnoside (II), kaempferol (III) and p-hydroxybenzoic acid (IV) by human intestinal flora.</p><p><b>CONCLUSION</b>The structure of kaempferitrin can be biotransformatedly converted by human intestinal flora.</p>